Micropropagation of Tarenaya rosea (Cleomaceae) from leaf explants

Abstract In vitro culture techniques are recognized as efficient strategies for large-scale plant production, well providing alternatives conservation. this study the micropropagation of Tarenaya rosea was established using petiole and foliar blade segments cultivated on MS medium with 6-benzyladenine (BA) and/or 6-furfurylaminopurine (KIN). The regeneration rate from explants evaluated after 30-days in culture, proliferation explant-derived shoots, reached four subcultures performed at culture. propagation occurred by both direct (DO) indirect (IO) organogenesis. highest rates DO (50% to 100%) were media containing only BA, while morphogenic calluses mainly formed BA+KIN. Explants BA showed presence small black nodules their surface, histological analysis revealed trichomes anthocyanin content. Elongation rooting growth regulator-free MS. Acclimatization around 80% achieved vitro-regenerated plants successfully maintained under field conditions. Results show significant morphogenetic potential T. leaf explants, when 4.4 µM a new alternative source material biotechnological conservation studies.